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Biological agents play a significant role in sewage treatment, with good removal effect, low sludge yield, good performance, and shorten the system start-up time, impact load resistance and stability enhancement.This paper describes the characteristics ofbiological agents and the effect of biological agents on wastewater denitrification was investigated through experiments, so as to provide effective basis support for the wide application of biological agents in the field of urban sewage treatment.The experiment showed that through reasonable addition of biological agents,the removal rates of COD,NH3-N and TNinlowpollutantsewage treatmentwere increased by 41%, 15% and 62% respectively.