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The antioxidant, antibacterial and antifungal properties of essential oils (EOs) of <i>Juniperus thurifera</i> L., a plant utilized in traditional, herbal medicine, were investigated. The EOs were extracted by use of a Clevenger apparatus and phytochemicals identified by gas chromatography coupled with mass spectrometry (GC/MS/MS). The antioxidant capacity of EOs of <i>J. thurifera</i> was determined by 2,2-diphenyl-1-picrylhydrazil (DPPH), total antioxidant capacity (TAC), and ferric reducing antioxidant power (FRAP). Antimicrobial activity of EOs of <i>J. thurifera</i> was determined against four fungal strains, <i>Candida albicans</i>; ATCC 10231, <i>Aspergillus niger</i>; MTCC 282, <i>Aspergillus flavus</i>; MTCC 9606 and <i>Fusarium oxysporum</i>; MTCC 9913 and four bacterial strains, <i>Staphylococcus aureus</i>; ATCC 6633, <i>Escherichia coli</i>; K12, <i>Bacillus subtilis</i>; DSM 6333, and <i>Pseudomonas aeruginosa</i>; CIP A22, by use of the disk diffusion method, and microdilution method used to determine the minimum inhibitory concentration (MIC). EOs of <i>J. thurifera</i> consisted of 31 compounds and were dominated by α-thujene (25%), elemol (12%) and muurolol (12%). Antioxidant activity recorded an IC₅₀ of 24 ± 0.71 µg/mL (DPPF), EC50 of 0.19 ± 0.01 mg/mL (FRAP), and 9.3 × 10² ± 38 mg EAA/g (TAC). The EOs of <i>J. thurifera</i> exhibited significant antibacterial activity against all bacterial strains under investigation, especially <i>P. aeruginosa</i>; CIP A22 with an inhibition diameter of 28 ± 1.5 mm and MIC of 4.8 × 10⁻² ± 0. 001 µg/mL. EOs of <i>J. thurifera</i> also exhibited significant antifungal activity against <i>C. albicans</i>; ATCC 10231 and <i>F. oxysporum</i>; MTCC 9913 with an activity of 21 ± 2.1 mm, 32 ± 2.3%, and MIC of 9.5 × 10⁻² ± 0.001 Bioactive molecules found in EOs of <i>J. thurifera</i> could be used as an alternative solution to antibiotics available on the market to combat microbial resistance.