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Objective. Nature has a significant impact on general well-being. However, till date, little psychophysiological evidence is available on the benefits of nature-based activities in adults. The primary goal of this study was to determine the physiological and psychological benefits of horticultural activity in adults. The participants were instructed to perform the making of a flower basket (horticultural activity) and a computer task (i.e., control activity) was compared. Methods. A total of 40 Chinese females (mean age 22.2 ± 0.9 years) participated in this experiment. The Blood pressure, the State-Trait Anxiety Inventory (STAI), and the electroencephalograms (EEGs) were used to measure the participants’ psychophysiological responses. Results. Analysis of the STAI data showed a lower anxiety score after performing the horticultural activity than after performing the control activity. Furthermore, in the EEG evaluation, variations in the brainwaves were observed after both activities. Conclusions. The study results suggest that horticultural activity induced physiological and psychological relaxation in adults.