Flavonoids from <i>Chionanthus retusus</i> (Oleaceae) Flowers and Their Protective Effects against Glutamate-Induced Cell Toxicity in HT22 Cells
oleh: Yeong-Geun Lee, Hwan Lee, Jae-Woo Jung, Kyeong-Hwa Seo, Dae Young Lee, Hyoung-Geun Kim, Jung-Hwan Ko, Dong-Sung Lee, Nam-In Baek
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2019-07-01 |
Deskripsi
The dried flowers of <i>Chionanthus retusus</i> were extracted with 80% MeOH, and the concentrate was divided into EtOAc, <i>n</i>-BuOH, and H<sub>2</sub>O fractions. Repeated SiO<sub>2</sub>, octadecyl SiO<sub>2</sub> (ODS), and Sephadex LH-20 column chromatography of the EtOAc fraction led to the isolation of four flavonols (<b>1</b>−<b>4</b>), three flavones (<b>5</b>−<b>7</b>), four flavanonols (<b>8</b>−<b>11</b>), and one flavanone (<b>12</b>), which were identified based on extensive analysis of various spectroscopic data. Flavonoids <b>4</b>−<b>6</b> and <b>8</b>−<b>11</b> were isolated from the flowers of <i>C. retusus</i> for the first time in this study. Flavonoids <b>1</b>, <b>2</b>, <b>5</b>, <b>6</b>, <b>8</b>, and <b>10</b>−<b>12</b> significantly inhibited NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) and glutamate-induced cell toxicity and effectively increased HO-1 protein expression in mouse hippocampal HT22 cells. Flavonoids with significant neuroprotective activity were also found to recover oxidative-stress-induced cell damage by increasing HO-1 protein expression. This article demonstrates that flavonoids from <i>C. retusus</i> flowers have significant potential as therapeutic materials in inflammation and neurodisease.