De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

oleh: Limin Shi, Chenxu Yan, Zhiqian Guo, Weijie Chi, Jingle Wei, Weimin Liu, Xiaogang Liu, He Tian, Wei-Hong Zhu

Format: Article
Diterbitkan: Nature Portfolio 2020-02-01

Deskripsi

Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantification.