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Titanium dioxide nanoparticles (TiO₂NPs) are found in a wide range of products such as sunscreen, paints, toothpaste and cosmetics due to their white pigment and high refractive index. These wide-ranging applications could result in direct or indirect exposure of these NPs to humans and the environment. Accordingly, conflicting levels of toxicity has been associated with these NPs. Therefore, the risk associated with these reports and for TiO₂NPs produced using varying methodologies should be measured. This study aimed to investigate the effects of various media on TiO₂NP properties (hydrodynamic size and zeta potential) and the effects of TiO₂NP exposure on human colorectal adenocarcinoma (Caco-2) epithelial cell viability, inflammatory and cell stress biomarkers and angiogenesis proteome profiles. The NPs increased in size over time in the various media, while zeta potentials were stable. TiO₂NPs also induced cell stress biomarkers, which could be attributed to the NPs not being cytotoxic. Consequently, TiO₂NP exposure had no effects on the level of inflammatory biomarkers produced by Caco-2. TiO₂NPs expressed some anti-angiogenic properties when exposed to the no-observed-adverse-effect level and requires further in-depth investigation.