AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis

oleh: Hong Jie Zhu, Bo Zhang, Wanqing Wei, Shuang He Liu, Lang Xiang, Jiapeng Zhu, Rui Hua Jiao, Yasuhiro Igarashi, Ghader Bashiri, Yong Liang, Ren Xiang Tan, Hui Ming Ge

Format: Article
Diterbitkan: Nature Portfolio 2022-08-01

Deskripsi

Macrocyclization is an important process in bioactive natural product synthesis. Here, the authors report on the study of a macrocyclic ring constructing enzyme in the biosynthesis of alchivemycin A and using gene deletion, biochemical assays and isotope labelling show the enzyme catalyses tandem dehydration and Michael-type addition.