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Background: Identifying End-Diastole (ED) and End-Systole (ES) frames is highly important in the process of evaluating cardiac function and measuring global parameters accurately, such as Ejection Fraction (EF), Cardiac Output (CO) and Stroke Volume. Objectives: The current study aimed to develop a new method based on measuring volume changes in Left Ventricle (LV) during cardiac cycle. Material and Methods: For this purpose, the Level Set method was used both in detecting endocardium border and quantifying cardiac function of all frames. Results: Demonstrating LV volumes displays ED and ES frames and the volumes used in calculating the required parameters. Conclusions: Since ES and ED frames exist in iso-volumic phases of the cardiac cycle with minimum and maximum values of LV volume signals, such peaks can be utilized in finding related frames.