Find in Library

To explore the mechanism by which <i>Akkermansia muciniphila</i> cell-free supernatant improves glucose and lipid metabolisms in <i>Caenorhabditis elegans</i>, the present study used different dilution concentrations of <i>Akkermansia muciniphila</i> cell-free supernatant as an intervention for with <i>Caenorhabditis elegans</i> under a high-glucose diet. The changes in lifespan, exercise ability, level of free radicals, and characteristic indexes of glucose and lipid metabolisms were studied. Furthermore, the expression of key genes of glucose and lipid metabolisms was detected by qRT-PCR. The results showed that <i>A. muciniphila</i> cell-free supernatant significantly improved the movement ability, prolonged the lifespan, reduced the level of ROS, and alleviated oxidative damage in <i>Caenorhabditis elegans</i>. <i>A. muciniphila</i> cell-free supernatant supported resistance to increases in glucose and triglyceride induced by a high-glucose diet and downregulated the expression of key genes of glucose metabolism, such as <i>gsy-1</i>, <i>pygl-1</i>, <i>pfk-1.1</i>, and <i>pyk-1,</i> while upregulating the expression of key genes of lipid metabolism, such as <i>acs-2</i>, <i>cpt-4</i>, <i>sbp-1</i>, and <i>tph-1,</i> as well as down-regulating the expression of the <i>fat-7</i> gene to inhibit fatty acid biosynthesis. These findings indicated that <i>A. muciniphila</i> cell-free supernatant, as a postbiotic, has the potential to prevent obesity and improve glucose metabolism disorders and other diseases.