A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy
oleh: Kanako Miyano, Katsuya Ohbuchi, Yuka Sudo, Kouichiro Minami, Toru Yokoyama, Masahiro Yamamoto, Miaki Uzu, Miki Nonaka, Seiji Shiraishi, Hiroaki Murata, Yoshikazu Higami, Yasuhito Uezono
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2020-08-01 |
Deskripsi
Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.