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Leptochilus decurrens is a small tropical fern of Polypodiaceae, but also a least concern folk medicinal fern. Its genome size is 153,753 bp with a pair of 21,664 bp inverted repeat regions (IR), a large single-copy (LSC) region of 84,395 bp and a small single-copy (SSC) region of 26,030 bp. A total of 133 genes were predicted, including 88 protein-coding genes, 37 tRNA genes and eight rRNA genes. The ML analysis reveals that L. decurrens is sister to Leptochilus hemionitideus forming monophyletic group closely related with polypodiaceous ferns. The work provides vigorous molecular data to further promote phylogenetics of Polypodiaceae.