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In the present paper changes of validity in Euler-Bernoulli's elementary theory of flexural vibration for homogeneous materials were tested with respect to changing severity and position of a single defect in wood. As an orthotropic material, wood has different material properties or strengths in different orthogonal directions. A set of absolutely clear specimens of oriental beech was chosen and hand drilled in different diameters (severities) at different relative distances from an end oriented in the R direction. A clear specimen showed a steady decrease in evaluated moduli of elasticity related to increasing mode numbers. After creating the defects, this steady decrease line showed some breakages. The slope breakages of modally evaluated elastic moduli in LT and LR vibrations are suggested as potential finger-prints of single hole defects in the specimen by considering the shape and rate of breakages in the decreasing lines. The recognition scenarios of slope breakages for defect severity and position are summarized.