Find in Library

Diarrhea is the most common issue in sheep farms, typically due to pathogenic <i>Escherichia coli</i> (<i>E. coli</i>) infections, such as <i>E. coli</i> F17. microRNA, a primary type of non-coding RNA, has been shown to be involved in diarrhea caused by pathogenic <i>E. coli</i>. To elucidate the profound mechanisms of miRNA in <i>E. coli</i> F17 infections, methods such as <i>E. coli</i> F17 adhesion assay, colony counting assay, relative quantification of bacterial <i>E. coli</i> fimbriae gene expression, indirect immune fluorescence (IF), Cell Counting Kit-8 (CCK-8), 5-ethynyl-2′-deoxyuridine (EdU), Western blotting (WB), and scratch assay were conducted to investigate the effect of miR-329b-5p overexpression/knock-down on <i>E. coli</i> F17 susceptibility of sheep intestinal epithelial cells (IECs). The findings indicated that miR-329b-5p enhances the <i>E. coli</i> F17 resistance of sheep IECs to <i>E.coli</i> F17 by promoting adhesion between <i>E. coli</i> F17 and IEC, as well as IEC proliferation and migration. In summary, miR-329b-5p plays a crucial role in the defense of sheep IECs against <i>E. coli</i> F17 infection, providing valuable insights into its mechanism of action.