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<i>C1orf74</i>, also known as URCL4, has been reported to have higher expression and be associated with poor prognosis in lung adenocarcinoma patients, and its role in regulation of the EGFR/AKT/mTORC1 pathway has been recently elucidated. In the current study, we used publicly available data and experimental validation of <i>C1orf74</i> gene expression and its association with prognosis in cervical cancer patients. qRT-PCR was performed using RNA from cervical cancer cell lines and twenty-five cervical cancer patients. Data from TNMplot revealed that mRNA expression of the <i>C1orf74</i> gene in primary tumor tissues, as well as metastatic tissues from cervical cancer patients, was significantly higher compared to normal cervical tissues. HPV-positive tumors had higher expression of this gene compared to HPV-negative tumors. qPCR analysis also demonstrated higher expression of <i>C1orf74</i> in HPV-positive cervical cancer cell lines and most cervical cancer patients. The promoter methylation levels of the <i>C1orf74</i> gene in cervical cancer tissues were lower compared to normal cervical tissues (<i>p</i> < 0.05). Collectively, our study indicates that higher expression of the <i>C1orf74</i> gene caused by hypomethylation of its promoter is associated with poor overall survival in cervical cancer patients. Thus, <i>C1orf74</i> is a novel prognostic marker in cervical cancer.