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Cassava is cultivated for two ends proposals: “sweet cassava” as fresh consumes and “industry cassava” as source of starch and farina. Landraces were used to discover “spontaneous mutations” and to develop evolutionary and breeding perspective of gene function. Genomic and Proteomic resources were obtained. Gene expression by RNA blot and Microarray analysis were performed to identify differentially expressed genes. A new sugary cassava was identified to be related to missing expression of BEI and a nonsense mutation in GBSSI gene leading to amylose free starch. A pink phenotype showed no expressionof CasLYB gene, and a yellow phenotype a down regulation of CasHYb. Proteomic analysis of carotenoid-protein complex togetherwith gene expression analysis of CAP4 revealed a heteroduplex double strand cDNA associated with high carotenoid content. GBSSIgene sequencing identified 22 haplotypes and large nucleotide diversity. Segregating populations by crossing differential biochemicalphenotypes and parents adapted to Cerrado’s Region were obtained.