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During the past decade, regenerative medicine has emerged as a key technology in thenext generation of medical care, and cell therapy and organ repair using stem cells havebecome very attractive options for regenerative medicine. The application of stem cells inregenerative medicine has required modified methods for isolation. Furthermore, the processof cell separation plays an important role in cell therapy and regenerative medicineusing stem cells. So, in this review, we compare different methods for the separation ofcells from bone marrow for transplantation to humans, with emphasis on the advantagesand disadvantages of each method.