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Detection of the hbl complex genes in Bacillus cereus isolated from cow raw milk in northwest of Iran
oleh: R. Alipour Banaei, Saman Mahdavi
Format: | Article |
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Diterbitkan: | University of Mosul, College of Veterinary Medicine 2020-05-01 |
Deskripsi
<em>Bacillus(B) cereus</em> is regarded as a major foodborne pathogen which is widely distributed in the nature. In addition, it plays an important role in the contamination of ready-to-eat and dairy products. <em>B. cereus</em> causes the two different types of food poisoning in human: the diarrheal and the emetic type. The aim of this study is detection of <em>hbl</em> complex genes in <em>B. cereus</em> isolated from cow raw milk in Northwest of Iran. In the present study, the number of the samples collected from cow raw milk were 120. All the isolates already had been identified phenotypically, and they were assessed for molecular confirmation by using the PCR method. <em>B. cereus</em> isolates were determined by detecting the <em>hbl</em> genes complex in the isolates. The result of this study showed that <em>B. cereus</em> were found in the raw milk samples 117 (97.5%) from the 120 samples. The frequency of the <em>hbl</em>A, <em>hbl</em>C, and <em>hbl</em>D genes found in <em>B. cereus</em> isolates were 105 (89.7%), 102 (87.1%), and 102 (87.1%) , respectively. 99 isolates (84.6%) harboured 3 tested genes simultaneously. 12 <em>B. cereus</em> isolates (10.3%) lacked these genes. The results of current study showed that <em>B. cereus</em> isolated from raw milk have high potential in causing food poisoning and therefore the use of the procedures to reduce the bacterial contamination during the processing of dairy product is required.