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This work aimed to reveal the heterotrophic nitrification and aerobic denitrification activities in a salt-tolerant strain, <i>Tritonibacter mobile</i> HN1, isolated from mariculture sludge by using a pure culture method. Strain HN1 showed the removal rates of ammonia, nitrite, nitrate, and total nitrogen of 98.22%, 100%, 95.71%, and 86.63%, respectively, with the carbon source of sodium citrate or sodium succinate, ratio of carbon to nitrogen of 15, salinity of 3%, temperature of 30 °C, shaking speed of 120 rpm, and pH of 8. The genes of <i>narG</i>, <i>narH</i>, <i>narI</i>, <i>nirS</i>, <i>norB</i>, <i>norC</i>, <i>nosZ</i>, <i>glnA</i>, and <i>GltB</i> were found in the genome sequence of strain HN1, confirming the pathways of nitrogen assimilation, heterotrophic nitrification, and aerobic denitrification. In addition, two nitrifying genes, <i>amo</i> and <i>nap</i>, were missing in the genome of <i>Tritonibacter mobile</i> HN1, indicating that HN1 may have novel genes for this function. This study showed that HN1 had the potential to remove nitrogen contaminants in saline environments and was the first <i>Tritonibacter mobilis</i> strain with heterotrophic nitrification and aerobic denitrification capabilities.