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Dairy cows assigned to four treatment groups were administered PGF2 ∝ analogue (Cloprostenol) on day 8 or day 25 post-partum (n=6 each), or antibiotic from day 1 to 5 post-partum (n=6), or kept as untreated control (n=6). Body condition score recorded at the time of calving was significantly different (p>0.05) between PG8 and PG25 treatment group. The monitoring of anovulatory follicles (>8mm) was performed by transrectal ultrasound scanning at weekly interval. Incidence of atretic follicles was significantly lower (p<0.01) in cows administered PGF2 ∝ on day 8 post-partum as compared to antibiotic and control group. In brief, PGF2 ∝ administration on day 8 post-partum can lead to lower incidence of anovulatory follicles and subsequently, improves the reproductive efficiency of dairy cows.