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Basic and theoretical research on processes such as reverse osmosis (RO) is essential in the fermentation industry to improve production efficiency and reduce cost. Here, we focus on the RO concentration of glucose solutions. We constructed a mathematic model that incorporates various membrane and experimental parameters to characterize the mass transfer process of RO membrane and validated the model output with experimental data. Calculation results were highly consistent with the experimental data, demonstrating that this model can be useful for predicting the RO concentration process.