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Background/purpose: Data for comparing effects of advanced platelet-rich fibrin (A-PRF+) and injectable platelet-rich fibrin (i-PRF) against Aggregatibacter actinomycetemcomitans (Aa) in subjects with differently periodontal conditions are scarce. This study aimed to compare the antimicrobial capacity of A-PRF+ and i-PRF obtained from subjects with or without periodontal diseases against the pathogenic bacteria Aa. Materials and methods: The number of red blood cells, platelets, and white blood cells on the blood samples of 60 individuals, including healthy subjects (n = 20), patients with gingivitis (n = 20), and patients with periodontitis (n = 20), were analyzed before preparing A-PRF+ and i-PRF. In addition, the in vitro antibacterial effect of the two platelet concentrates was evaluated by using the agar diffusion test and a minimum inhibitory concentration (MIC) experiment. Results: I-PRF exhibited a significantly better antibacterial effect than A-PRF+ within the gingivitis and periodontitis groups, with a more expansive zone of inhibition and a lower MIC. Among the studied groups, the A-PRF+ and i-PRF collected from the periodontitis group inhibited Aa significantly more compared with the gingivitis and healthy groups. Conclusion: Although both A-PRF+ and i-PRF exhibited an antibacterial effect against Aa through the zone of inhibition and MIC tests, in the gingivitis and periodontitis groups, i-PRF exhibited better antibacterial activity than A-PRF+, and PRF products from the periodontitis group had greater effects against Aa than PRF products from the two other groups.