Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
oleh: Sergei Svitashev, Christine Schwartz, Brian Lenderts, Joshua K. Young, A. Mark Cigan
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-11-01 |
Deskripsi
Genome editing in plants typically requires the expression of Cas9 and guide RNA from stably transformed plasmid DNA. Here, the authors show that successful editing can be achieved after delivery of the Cas9-guide RNA complex as a ribonucleoprotein to maize embryos via biolistics.