Find in Library

Immune response of day-old chicks infected with <i>Salmonella</i> Enteritidis PT4 and preventive administration of <i>Enterococcus faecium</i> AL41 were studied using hematology and flow cytometry of immunocompetent cells in blood, cecum, bursa and spleen for 11 days, and included 220 animals divided into four groups (n = 55). <i>E. faecium</i> AL41 was administered for 7 days to EF and EFSE groups and on day 4 SE and EFSE groups were infected with <i>Salmonella</i> Enteritidis. Values of monocytes at 4 dpi significantly increased in EFSE and lymphocytes at 7 dpi in EF groups. Blood CD3, CD4, CD8 and IgM lymphocytes improved in EF and EFSE groups and IgA in EF group at 4 dpi. Phagocytic activity of probiotic groups was improved in both samples. Cecal IEL and LPL lymphocytes showed at 7 dpi stimulation of CD3, CD4 and CD8 subpopulations in probiotic groups, especially in EFSE group, IgA IEL and IgA with IgM LPL in EF groups. Bursa Fabricii at 7 dpi presented overstimulation of IgG subpopulation in SE group, spleen CD3 and CD8 in EF and EFSE groups. <i>E. faecium</i> AL41 revealed the protective effect and positive influence on the local and systemic immune response in <i>Salmonella</i> Enteritidis PT4 infected chickens.