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Nanoscopy of bacterial cells immobilized by holographic optical tweezers
oleh: Robin Diekmann, Deanna L. Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser
Format: | Article |
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Diterbitkan: | Nature Portfolio 2016-12-01 |
Deskripsi
Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.