Nanoscopy of bacterial cells immobilized by holographic optical tweezers

oleh: Robin Diekmann, Deanna L. Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser

Format: Article
Diterbitkan: Nature Portfolio 2016-12-01

Deskripsi

Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.