Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks

oleh: Holly A. Rees, Wei-Hsi Yeh, David R. Liu

Format: Article
Diterbitkan: Nature Portfolio 2019-05-01

Deskripsi

Here the authors fuse hRad51 and variants thereof to Cas9 nickase to facilitate homology-directed repair without generating double strand breaks, minimizing indel formation and off-target editing. This tool represents progress towards the goal of performing HDR without an excess of undesired side products.