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<p>A total number of 3690 urine samples were processed in the Microbiology Department during one year period, of which 589 were culture positive. Among them <em>Escherichia</em> <em>coli</em> (45%), <em>Klebsiella spp.</em> (15%), <em>Enterobactor cloacae </em>(12%), <em>Staphylococcus aureus </em>(6%),<em> Enterococcus faecalis </em>(9.8%), <em>Pseudomonas</em> <em>spp</em>. (9%), coagulase negative<em> Staphylococci </em>(1.1%) and <em>Acinetobactor spp</em>.(2.1%)<em> </em>were the predominant organisms.31.6% <em>Escherichia coli</em> and 20.4% <em>Klebsiella pneumoniae</em> were Extended spectrum β lactamase (ESBL) producers. They  also showed multidrug resistance to quinolones and aminoglycosides. Carbapenemase producers were also increased among ESBL producers.</p><p>DOI: <a href="http://dx.doi.org/10.4038/sljid.v2i1.3716">http://dx.doi.org/10.4038/sljid.v2i1.3716</a></p><p><em>Sri Lankan Journal of Infectious Diseases </em>Vol.2(1) 2012: 36-41</p>