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This paper presents the research protocol for a Phase 2 randomized controlled trial of the Fluency into Comprehension program in primary schools. The program is a workforce development program that supports teachers and teaching assistants develop and deliver targeted reading fluency and comprehension instruction to children aged 7–9. The protocol outlines a research design to assess whether the program delivered over approximately 12 weeks improves reading outcomes, in a sample of up to 192 children from 12 schools in four English districts with high socio-economic disadvantage. The outcome measures are two reading standardized tests. A process evaluation will measure fidelity and potential for scale-up.