Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
oleh: Youngbin Lim, So Young Bak, Keewon Sung, Euihwan Jeong, Seung Hwan Lee, Jin-Soo Kim, Sangsu Bae, Seong Keun Kim
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-11-01 |
Deskripsi
In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.