Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
oleh: Johan O. L. Andreasson, Andrew Savinov, Steven M. Block, William J. Greenleaf
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-04-01 |
Deskripsi
RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.