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The high-affinity K⁺ transporter (HAK) family, the most prominent potassium transporter family in plants, which involves K⁺ transport, plays crucial roles in plant responses to abiotic stresses. However, the <i>HAK</i> gene family remains to be characterized in quinoa (<i>Chenopodium quinoa</i> Willd.). We explored HAKs in quinoa, identifying 30 members (<i>CqHAK1</i>–<i>CqHAK30</i>) in four clusters phylogenetically. Uneven distribution was observed across 18 chromosomes. Furthermore, we investigated the proteins’ evolutionary relationships, physicochemical properties, conserved domains and motifs, gene structure, and <i>cis</i>-regulatory elements of the CqHAKs family members. Transcription data analysis showed that <i>CqHAKs</i> have diverse expression patterns among different tissues and in response to abiotic stresses, including drought, heat, low phosphorus, and salt. The expressional changes of <i>CqHAKs</i> in roots were more sensitive in response to abiotic stress than that in shoot apices. Quantitative RT-PCR analysis revealed that under high saline condition, <i>CqHAK1</i>, <i>CqHAK13</i>, <i>CqHAK19</i>, and <i>CqHAK20</i> were dramatically induced in leaves; under alkaline condition, <i>CqHAK1</i>, <i>CqHAK13</i>, <i>CqHAK19</i>, and <i>CqHAK20</i> were dramatically induced in leaves, and <i>CqHAK6</i>, <i>CqHAK9</i>, <i>CqHAK13</i>, <i>CqHAK23</i>, and <i>CqHAK29</i> were significantly induced in roots. Our results establish a foundation for further investigation of the functions of <i>HAKs</i> in quinoa. It is the first study to identify the HAK gene family in quinoa, which provides potential targets for further functional study and contributes to improving the salt and alkali tolerance in quinoa.