Proteomic analysis of whey from bovine colostrum and mature milk

oleh: Luciana Pacheco Golinelli, Carlos Adam Conte-Junior, VĂ¢nia Margaret Flosi Paschoalin, Joab Trajano Silva

Format: Article
Diterbitkan: Instituto de Tecnologia do ParanĂ¡ (Tecpar) 2011-08-01

Deskripsi

The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.