Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
oleh: Inga Rødahl, James Gotley, Stacey B. Andersen, Meihua Yu, Ahmed M. Mehdi, Angelika N. Christ, Emma E. Hamilton-Williams, Ian H. Frazer, Samuel W. Lukowski, Janin Chandra
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-12-01 |
Deskripsi
Summary: Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species.For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).