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<i>Bartonella bacilliformis (B. bacilliformis)</i>, <i>Bartonella henselae (B. henselae)</i>, and <i>Bartonella quintana (B. quintana)</i> are bacteria known to cause verruga peruana or bacillary angiomatosis, vascular endothelial growth factor (VEGF)-dependent cutaneous lesions in humans. Given the bacteria’s association with the dermal niche and clinical suspicion of occult infection by a dermatologist, we determined if patients with melanoma had evidence of <i>Bartonella</i> spp. infection. Within a one-month period, eight patients previously diagnosed with melanoma volunteered to be tested for evidence of <i>Bartonella</i> spp. exposure/infection. Subsequently, confocal immunohistochemistry and PCR for <i>Bartonella</i> spp. were used to study melanoma tissues from two patients. Blood from seven of the eight patients was either seroreactive, PCR positive, or positive by both modalities for <i>Bartonella</i> spp. exposure. Subsequently, <i>Bartonella</i> organisms that co-localized with VEGFC immunoreactivity were visualized using multi-immunostaining confocal microscopy of thick skin sections from two patients. Using a co-culture model, <i>B. henselae</i> was observed to enter melanoma cell cytoplasm and resulted in increased vascular endothelial growth factor C (VEGFC) and interleukin 8 (IL-8) production. Findings from this small number of patients support the need for future investigations to determine the extent to which <i>Bartonella</i> spp. are a component of the melanoma pathobiome.