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Objective To study the effect of tetrandrine (TET) on oral squamous cell carcinoma cell line Tca8113 and its influence against the expression of β-catenin.Methods CCK-8 method was used to detect the effect of TET on Tca8113 by different dose and time. Western blot assay was used to detect the expression of β-catenin after treatment by TET meanwhile the distribution of the β-catenin treated by TET was detected by the immunofluorescence method.Results With the increasing dose of TET, the inhibition rate against Tca8113 was raising (P < 0.05). The half maximal inhibitory concentration (IC50) value of TET against Tca8113 was 64 μmol/L. With this concentration, the inhibition rate against Tca8113 exhibited a time-dependent manner. The results of western blot revealed that the expression of β-catenin was significantly inhibited after treatment by TET (P < 0.05). The results of immunofluorescence method showed that the β-catenin protein mainly located at nucleus in Tca8113, while after treatment by TET, the β-catenin protein mainly located at cellular membrane and cytoplasm in a diffused distribution manner.Conclusion Our data suggest that TET can inhibit the cell growth of oral squamous cell carcinoma, and influence the cellular distribution of β-catenin protein in tumor cells.