Find in Library

Screening and identifying the active compounds in foods are important for the development and utilization of functional foods. In this study, the anti-enteritis activity of ethanol extract from <i>Camellia oleifera</i> oil (<i>PECS</i>) was quickly evaluated using a Smurf Drosophila model and the metabolomics approach, combined with molecular docking techniques, were performed to rapidly screen and identify compounds with potential anti-enteritis activity in <i>PECS</i>. <i>PECS</i> showed good anti-enteritis activity and inhibited the activity of 5-lipoxygenase (LOX), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS). In particular, wighteone and <i>p</i>-octopamine were newly identified in <i>C. oleifera</i> oil and were proven to have good anti-enteritis activity. The inhibitory activity of kaempferitrin (IC₅₀ = 0.365 mmol L⁻¹) was higher than that of wighteone (IC₅₀ = 0.424 mmol L⁻¹) and <i>p</i>-octopamine (IC₅₀ = 0.402 mmol L⁻¹). Of note, the IC₅₀ value of salazosulfapyridine was 0.810 mmol L⁻¹. Inhibition of LOX activity is likely one of the anti-enteritis mechanisms of <i>PECS</i>. These new findings lay the foundation for further investigations into the underlying mechanisms of anti-enteritis activity in <i>C. oleifera</i> oil.