An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
oleh: Hongmin Cai, Hebang Yao, Tingting Li, Cedric A. J. Hutter, Yanfang Li, Yannan Tang, Markus A. Seeger, Dianfan Li
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-12-01 |
Deskripsi
In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification.