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Kefir is a mixed fermented product with numerous attributed health benefits due to presence of a complex culture composed of bacteria and yeasts in an exopolysaccharide matrix. This work aimed at isolating and identifying yeast species from two types of traditional kefir grains and establishing some potential probiotic properties including survival in the gastrointestinal tract, auto-aggregation, hydrophobicity and hydrolytic enzymes production. All the isolates showed good survival rates in simulated gastrointestinal tract solution, with <0.5 log10 reduction. Kluyveromyces lactis was characterized with a high level of hydrophobicity (88.75%) but moderate auto-aggregation whereas S. unisporus showed moderate hydrophobicity and auto-aggregation. Indicator enteric bacteria adhered onto both viable and non-viable yeast isolates and controls. In comparison to Saccharomyces boulardii strains used as controls, both kefir yeast strains showed low alpha hemolytic and proteolytic activities, but exhibited no phospholipase activity. Kluyveromyces lactis and Saccharomyces unisporus isolated, were identified on the basis of 26s rDNA and ITS region sequencing. Overall, the yeast isolates showed some potential probiotic properties.