Find in Library

Summary: Despite effective treatment, HIV can persist in latent reservoirs, which represent a major obstacle toward HIV eradication. Targeting and reactivating latent cells is challenging due to the heterogeneous nature of HIV-infected cells. Here, we used a primary model of HIV latency and single-cell RNA sequencing to characterize transcriptional heterogeneity during HIV latency and reactivation. Our analysis identified transcriptional programs leading to successful reactivation of HIV expression. : HIV latency hampers HIV cure. The shock-and-kill strategy aims at reactivating HIV expression to purge the latent reservoir of HIV-infected cells. However, latently infected cells do not respond equally to stimulation. Golumbeanu et al. use single-cell RNA-seq to characterize cell heterogeneity and identify transcriptional features leading to reactivation success. Keywords: scRNA-seq, transcriptome, heterogeneity, inducible cell, signature, single cell, HIV, latency, reactivation, cure