Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
oleh: Nilmani Singh, Adriana Reyes-Ordoñez, Michael A. Compagnone, Jesus F. Moreno, Benjamin J. Leslie, Taekjip Ha, Jie Chen
| Format: | Article |
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| Diterbitkan: | Nature Portfolio 2021-07-01 |
Deskripsi
Pleckstrin homology (PH) domains are presumed to bind phosphoinositides (PIPs) but only few specific interactions are known. Using TIRF microscopy based assays, functional studies and an experimentally validated prediction algorithm, the authors show that specific PIP binding is widespread among human PH domains.