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<p>Background. A change in retinal thickness caused by several ocular diseases can be measured quantitatively by the retinal thickness analyser (RTA). The principle of RTA is based on projecting a laser slit (540 nm) onto the retina and viewing its intersection with the retina. The separation between the reflections from the vitreoretinal and the chorioretinal interface is a measure of the retinal thickness. By scanning the posterior pole and/or peripapillary retina, the intersections of the laser slit with the retina are presented as multiple optical cross sections and as colour-coded retinal thickness maps.</p><p>Conclusions. In the article the application of the new method in glaucoma and other retinal diseases is presented. Scanning RTA is a non-invasive method and provides quantitative information that may be useful in the diagnosis and monitoring of glaucoma and a variety of retinal diseases in clinical practice.<br /><br /></p>