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An experiment was conducted under field condition to evaluate the effects of pure or combinations of biofertilizers on agronomic and quality criteria of Hyssop (Hyssopus officinalis), a medicinal and aromatic plant from Lamiaceae family at Research Station of Faculty of Agriculture, Ferdowsi University of Mashhad, during years of 2006 and 2007. A complete randomized block design with three replications was used. Fertilizers containing Azospirillum/Azotobacter (Nitroxin), Azospirillum/ Bacillus subtilis/ Pseudomonas fluorescens (Super Nitro Plus), Glomus intraradices (Mycorrhizal inoculant), Pseudomonas fluorescens, Glomus intraradices / Pseudomonas fluorescens, Azospirillum/ Azotobacter/ Glomus intraradices / Pseudomonas fluorescens and control. Results indicated that in general, application of biofertilizers enhanced yield and other plant criteria in this plant. Plant performanced better with application of Super Nitro Plus and a mixture of Glomus intraradices and Pseudomonas fluorescens in terms of most plant criteria. However, in the second year plants performed better in response to biofertilizers and this is due to the nature of plant with regard to prenniality.