A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
oleh: Hai Yu, Hongliang Huang, Da Li, Yang Liu, Fenping Shen, Jun Zhou, Qingqing Wang, Guping Tang
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2008-11-01 |
Deskripsi
A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.