Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9
oleh: Chun-Lin Liu, Ching-Ying Huang, Hung-Chih Chen, Huai-En Lu, Patrick C.H. Hsieh, Jia-Jung Lee
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2020-05-01 |
Deskripsi
We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripotency, and differentiation capacity into three germ layers.