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Production of a novel multi-epitope vaccine based on outer membrane proteins of Klebsiella pneumoniae
oleh: Tayebeh Farhadi, Zeinab Karimi, Younes Ghasemi, Navid Nezafat, Shiva Hemmati, Nasrollah Erfani
Format: | Article |
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Diterbitkan: | Shiraz University of Medical Sciences 2015-07-01 |
Deskripsi
<p><em>Klebsiella pneumoniae</em> is a hospital-acquired pathogen that leads to various infections. Hence, efforts to develop an effective vaccine against that pathogen are well documented. Our interest is the production of the previously designed multi-epitope vaccine construct against the <em>K. pneumoniae</em> in a prokaryotic host. Therefore, a new construct containing the nucleotide sequence of the novel vaccine was successfully expressed in<em> Escherichia coli</em> and then purified by Ni-NTA spin column. The purified recombinant protein can be considered as potential vaccine candidate for wet-laboratory analysis aiming to fight <em>K pneumoniae</em> .</p>