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Nanobiotechnology is a broad science that provides nanomaterials to solve many medical, agricultural, engineering, biological, and chemical problems. Nanoparticles (NPs) are nanomaterials with zero dimensions. Due to its numerous applications in a variety of scientific fields, semiconductor NPs synthesis has received a lot of attention in recent times. There are three methods that can be used to make NPs including chemical, biological, and physical methods. ZnO-NPs has been synthesized by the biological method in this study. The green husk of <i>Juglans regia</i> aqueous extract was used to make NPs using the biological method. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and UV-Visible spectroscopy were used to examine the NPs’ properties. The biosynthesized ZnO-NPs have a strong SPR band at 360 nm, as demonstrated by the UV-Vis analysis results. These NPs are crystalline. In addition, the results of the DLS analysis showed that the hydrodynamic diameter of 90% of the biosynthesized zinc oxide is less than 292.55 nm. Furthermore, the TEM analysis confirmed that these NPs have spherical and ellipsoidal shapes.