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The complete mitochondrial genome (mitogenome) of Opisthotropis andersonii was first determined using illumina next-generation sequencing. The total length of genome was 18,297bp with a slightly AT bias (59.57%), and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, 2 control regions, and an origin of light-strand replication. All genes are distributed on the H-strand, except ND6 gene. A phylogenetic tree was constructed based on the 13 PCG sequences of O. andersonii and other related snakes to analyze their phylogenic relationship, exhibiting the closest relationship with Thermophis baileyi and Nerodia sipedon.