Internal guide RNA interactions interfere with Cas9-mediated cleavage
oleh: Summer B. Thyme, Laila Akhmetova, Tessa G. Montague, Eivind Valen, Alexander F. Schier
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-06-01 |
Deskripsi
While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.