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It is long-cherished to develop rapid approaches for the identification and quantification of pesticide and its metabolites. Carbofuran (CBF) and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are highly toxic to humans and non-target organisms, which makes it mandatory for the detection of their residues in foods and the environment in many countries. Herein, a colloidal gold-strip assay based on a broad-specific monoclonal antibody (mAb) for simultaneous determination of CBF and 3-OH-CBF was developed. The colloidal gold-strips have a runtime within 5 min without complex sample pretreatment and perform a cut-off limit of detection (LOD) of 7–10 ng/mL for both carbofuran and 3-OH-CBF. The developed portable strip assay is a versatile and robust tool for the rapid and simultaneous detection of CBF and 3-OH-CBF in water samples as well as for the determination of illegal addition of CBF in other pesticide preparations.