Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
oleh: Manuel Nieto-Domínguez, Beatriz Fernández de Toro, Laura I. de Eugenio, Andrés G. Santana, Lara Bejarano-Muñoz, Zach Armstrong, Juan Antonio Méndez-Líter, Juan Luis Asensio, Alicia Prieto, Stephen G. Withers, Francisco Javier Cañada, María Jesús Martínez
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-09-01 |
Deskripsi
Thioglycoligases have proved useful for bonding carbohydrates to non-sugar acceptors, however, the scope of these biocatalysts is usually limited. Here, the authors engineer a xylosidase into a thioglycoligase with the ability to form O-, N-, S- and Se- glycosides together with sugar esters and phosphoesters.