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The aim of this study was to investigate the antifungal and antioxidant activities of <i>Chamaecyparis obtusa</i> (<i>C. obtusa</i>) extract (COE) against <i>Candida albicans</i> (<i>C. albicans</i>). Methods: The antioxidant activity was determined using three methods based on 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) scavenging, total polyphenol measurement, and total flavonoid measurement. In addition, the survival rate of human keratinocytes (HaCaT) was checked to confirm their stability. A one-way ANOVA also confirmed the antifungal activity of COE against <i>C. albicans</i> and its proliferative effect on HaCaT cells. In addition, to confirm the difference between each group, the Tukey HSD test was performed as a post hoc analysis, and the significance level was set at 0.05 as a two-tailed test. Results: COE was found to contain 424.150 mg GAE/g of polyphenols, 127.566 mg CE/g of flavonoids, and 83.594% of radical scavenging activity. Furthermore, 30 mg/mL of this extract exhibited complete antifungal activity. In addition, a noticeable killing effect on <i>C. albicans</i> was observed as the concentration of the extract increased. For instance, at a 2.5 mg/mL dosage of COE, HaCaT cells were safe, but <i>C. albicans</i> showed a distinct antifungal effect. Conclusions: COE has antioxidant and antifungal activity, so it has a high potential as an effective natural antifungal agent. Therefore, oral gargle containing COE is expected to be a safe and effective treatment in oral candidiasis in clinical practice.